Thanks Steve, yes I did change the output tubes around. When I bought the amp it still had the original Luxman branded Golden Dragon EL34 and 12AU7 tubes, the 12BH7A's had been changed for Philips. Not really knowing the history of the amp I decided to invest in new tubes and bought some Shuguang Treasure EL34, NOS Mullard 12AU7WA and RCA 12BH7A.Thanks Myles also, I checked the fuse's but there isn't one for each tube, what fuse's there are were ok. It was a resistor Steve thanks, not on the cathode but the screen grid.
Hopefully that's all it is, pin four on all four tubes are linked together via 100 ohm resistors, one for each tube. Out of the four the resistor for the tube with no bias was open circuit, two others were ok and one was out of spec at 120 ohm. I've ordered better quality 1% Tantalum resistors from Partsconnexion.com to replace all four. It will probably be a couple of weeks before I get them, thanks again for all the help and suggestions.
John.